ABSTRACT
5'-nucleotidase [5'-NT] and Gamma glutamyl transferase [GGT] activity was measured in the tissues of four 2-6 years old donkeys; four 6-9 month old castrated male goats and four 6-9 month old male calves. In donkeys the lung is the richest source of the enzyme 5'-NT, whereas in goats the liver contains a higher activity of the enzyme compared with other species. Generally the values of 5'-NT activities in tissues of calves and goats are higher than those of donkeys. GGT is mainly located in the kidneys, pancreas and liver of all the species studied. 20-25% loss in activity of GGT occurred after thawing and freezing of tissues for 2 weeks whereas more than 50% loss in activity of the enzyme occurred in four weeks period. The fall in the activity of 5'-NT in tissues was not consistent in all tissues during the first two weeks, but after four weeks period 50% loss of activity was observed
Subject(s)
Animals , Goats/physiology , Cattle/physiology , 5'-Nucleotidase/chemistry , gamma-Glutamyltransferase/chemistry , FreezingABSTRACT
Two procedures were adopted for determination of cefotaxime sodium in its pure and dosage forms. In the first procedure cefotaxime sodium was treated with sodium nitrite [1%, 1 ml] in the presence of hydrochloric acid [0.5%, 1 ml]. The developed red color exhibits an absorption maximum at 505 nm. Linear correlations were obtained between the absorbance of nitroso product and drug concentration over the range 80-240 mug ml-1. Upon rendered the medium alkaline with sodium hydroxide solution [1%, 1 ml], the red color was changed into violet color. The produced violet color has an absorption maximum at 563 nm and linearity in the range 80-160 mug ml-1 of cefotaxime sodium. In the second method, the utility of N-bromosuccinimide [NBS] and N-chlorosuccinimide [NCS] in estimation of cefotaxime sodium has been investigated. The study of reaction conditions revealed that, l mole of cefotaxime sodium reacts with 3 moles of NBS or NCS. The optimum time for complete the reaction was found to be 20 minutes. The method permits the determination of 1.5913 mg of cefotaxime sodium